Kevin Pfleger

Professor

  • The University of Western Australia (M519), 35 Stirling Highway,

    6009 Perth

    Australia

  • 2207 Citations
  • 23 h-Index
20022022
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Research Output 2002 2019

2019
1 Citation (Scopus)

Complex Formation between VEGFR2 and the β2-Adrenoceptor

Kilpatrick, L. E., Alcobia, D. C., White, C. W., Peach, C. J., Glenn, J. R., Zimmerman, K., Kondrashov, A., Pfleger, K. D. G., Ohana, R. F., Robers, M. B., Wood, K. V., Sloan, E. K., Woolard, J. & Hill, S. J., 20 Jun 2019, In : Cell Chemical Biology. 26, 6, p. 830-841.e9

Research output: Contribution to journalArticle

Open Access
Adrenergic Receptors
Intracellular Signaling Peptides and Proteins
Bioluminescence
Endosomes
Energy Transfer

Detection and characterisation of GPCR heteromers using NanoBRET ligand binding

Johnstone, E. K., See, H. B., Abhayawardana, R. S., Song, A., Rosengren, K. J., Hill, S. J. & Pfleger, K. D., Aug 2019, In : British Journal of Pharmacology. 176, 16, p. 3046-3046 1 p.

Research output: Contribution to journalAbstract/Meeting Abstract

Disease-associated missense variants in ZBTB18 disrupt DNA binding and impair the development of neurons within the embryonic cerebral cortex

Hemming, I. A., Clément, O., Gladwyn-Ng, I. E., Cullen, H. D., Ng, H. L., See, H. B., Ngo, L., Ulgiati, D., Pfleger, K. D. G., Agostino, M. & Heng, J. I. T., 21 May 2019, In : Human Mutation. 15 p.

Research output: Contribution to journalArticle

Cerebral Cortex
Neurons
DNA
Zinc Fingers
Brain Diseases

NanoBRET: The Bright Future of Proximity-Based Assays

Dale, N. C., Johnstone, E. K. M., White, C. W. & Pfleger, K. D. G., 26 Mar 2019, In : Frontiers in Bioengineering and Biotechnology. 7, MAR, 13 p., 56.

Research output: Contribution to journalReview article

Open Access
3 Citations (Scopus)

NanoBRET ligand binding at a GPCR under endogenous promotion facilitated by CRISPR/Cas9 genome editing

White, C. W., Johnstone, E. K. M., See, H. B. & Pfleger, K. D. G., Feb 2019, In : Cellular Signalling. 54, p. 27-34 8 p.

Research output: Contribution to journalArticle

Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis

Pickering, R., Tikellis, C., Rosado, C., Tsorotes, D., Dimitrropoulos, A., Smith, M., Huet, O., Seeber, R., Abhayawardana, R., Johnstone, E., Golledge, J., Wang, Y., Jandeleit-Dahm, K., Cooper, M., Pfleger, K. & Thomas, M. C., 2 Jan 2019, In : The Journal of clinical investigation. 129, 1, p. 406-421 CI99987.

Research output: Contribution to journalArticle

Angiotensins
Transcriptional Activation
Atherosclerosis
Inflammation
Angiotensin II
2018
1 Citation (Scopus)

BRET-based assay to monitor EGFR transactivation by the AT1R reveals Gq/11 protein-independent activation and AT1R-EGFR complexes

O'Brien, S. L., Johnstone, E. K. M., Devost, D., Conroy, J., Reichelt, M. E., Purdue, B. W., Ayoub, M. A., Kawai, T., Inoue, A., Eguchi, S., Hébert, T. E., Pfleger, K. D. G. & Thomas, W. G., 1 Dec 2018, In : Biochemical Pharmacology. 158, p. 232-242 11 p.

Research output: Contribution to journalArticle

Gq-G11 GTP-Binding Protein alpha Subunits
Bioluminescence
Energy Transfer
Epidermal Growth Factor Receptor
Energy transfer

Experiential Training Courses for Medical Technology and Pharmaceutical Translation

Seeber, R. & Pfleger, K., 28 Nov 2018.

Research output: Contribution to conferencePoster

Pharmaceutical Technology
Ecosystem
Biomedical Research
Aptitude
Organized Financing
141 Downloads (Pure)

Investigating microRNA-7 as a potential therapeutic in cancer

Horsham, J., 2018, (Unpublished)

Research output: ThesisDoctoral Thesis

File
MicroRNAs
Neoplasms
Nanoparticles
Therapeutics
Second Primary Neoplasms
1 Citation (Scopus)

Investigation of interactions between TLR2, MyD88 and TIRAP by bioluminescence resonance energy transfer is hampered by artefacts of protein overexpression

Sampaio, N. G., Kocan, M., Schofield, L., Pfleger, K. D. G. & Eriksson, E. M., 1 Aug 2018, In : PLoS One. 13, 8, e0202408.

Research output: Contribution to journalArticle

Open Access
Bioluminescence
bioluminescence
Energy Transfer
energy transfer
Artifacts
2017
11 Citations (Scopus)

Disease-associated mutations in RP58 disrupt its neuronal functions through a mechanism involving transcriptional regulation

Hemming, I. A., Clement, O., Gladwyn-Ng, I. E., Ng, H. L., See, E., Ngo, L., Ulgiati, D., Pfleger, K. D. G. & Heng, J. I. -T., Aug 2017, In : Journal of Neurochemistry. 142, p. 90-90 1 p.

Research output: Contribution to journalAbstract/Meeting Abstract

13 Citations (Scopus)

Key determinants of selective binding and activation by the monocyte chemoattractant proteins at the chemokine receptor CCR2

Huma, Z. E., Sanchez, J., Lim, H. D., Bridgford, J. L., Huang, C., Parker, B. J., Pazhamalil, J. G., Porebski, B. T., Pfleger, K. D. G., Lane, J. R., Canals, M. & Stone, M. J., 23 May 2017, In : Science Signaling. 10, 480, eaai8529.

Research output: Contribution to journalArticle

Monocyte Chemoattractant Proteins
Chemokine Receptors
Chemokines
Chemical activation
Chemokine CCL2
15 Citations (Scopus)

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

White, C. W., Vanyai, H. K., See, H. B., Johnstone, E. K. M. & Pfleger, K. D. G., 9 Jun 2017, In : Scientific Reports. 7, 1, 3187.

Research output: Contribution to journalArticle

Open Access
Clustered Regularly Interspaced Short Palindromic Repeats
Energy Transfer
Genome
G-Protein-Coupled Receptors
Proteins
2016
39 Citations (Scopus)

Fluorescence- and bioluminescence-based approaches to study GPCR ligand binding

Stoddart, L. A., White, C., Nguyen, K., Hill, S. & Pfleger, K., 1 Oct 2016, In : British Journal of Pharmacology. 173, 20, p. 3028-3037 10 p.

Research output: Contribution to journalArticle

Open Access
Fluorescence
Ligands
Fluorescence Resonance Energy Transfer
Pharmacology
Fluorescence Polarization
95 Citations (Scopus)

G Protein-Coupled Receptor Heteromers

Gomes, I., Ayoub, M. A., Fujita, W., Jaeger, W., Pfleger, K. & Devi, L. A., 2016, In : Annual Review of Pharmacology and Toxicology. 56, p. 403-425

Research output: Contribution to journalArticle

G-Protein-Coupled Receptors
Physiological Phenomena
Pathologic Processes
Membrane Proteins
Pharmacology
23 Citations (Scopus)

i-bodies, human single domain antibodies that antagonize chemokine receptor CXCR4

Griffiths, K., Dolezal, O., Cao, B., Nilsson, S. K., See, E. B., Pfleger, K. D. G., Roche, M., Gorry, P. R., Pow, A., Viduka, K., Lim, K., Lu, B. G. C., Chang, D. H. C., Murray-Rust, T., Kvansakul, M., Perugini, M. A., Dogovski, C., Doerflinger, M., Zhang, Y., Parisi, K. & 3 othersCasey, J. L., Nuttall, S. D. & Foley, M., 2016, In : Journal of Biological Chemistry. 291, 24, p. 12641-12657 17 p.

Research output: Contribution to journalArticle

Single-Domain Antibodies
Chemokine Receptors
Stem cells
Human Body
Hematopoietic Stem Cell Mobilization
10 Citations (Scopus)

Mutations of vasopressin receptor 2 including novel L312S have differential effects on trafficking

Tiulpakov, A., White, C., Abhayawardana, R., See, H. B., Chan, A., Seeber, R., Heng, J., Dedov, I., Pavlos, N. & Pfleger, K., 1 Aug 2016, In : Molecular Endocrinology. 30, 8, p. 889-904

Research output: Contribution to journalArticle

Open Access
Vasopressin Receptors
Mutation
Hyponatremia
Nephrogenic Diabetes Insipidus
Natriuresis
2015
78 Citations (Scopus)

Application of BRET to monitor ligand binding to GPCRs

Stoddart, L. A., Johnstone, E., Wheal, A. J., Goulding, J., Robers, M. B., Machleidt, T., Wood, K. V., Hill, S. & Pfleger, K., Jul 2015, In : Nature Methods. 12, 7, p. 661-663 3 p.

Research output: Contribution to journalArticle

Open Access
Bioluminescence
Energy Transfer
G-Protein-Coupled Receptors
Energy transfer
Luminescent Proteins
3 Citations (Scopus)

Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling

Johnstone, E. & Pfleger, K., 2015, G Protein-Coupled Receptors in Drug Discovery. Filizola, M. (ed.). 2nd ed. New York: Humana Press, Vol. 1335. p. 191-204 101. (Methods in Molecular Biology).

Research output: Chapter in Book/Conference paperChapter

19 Citations (Scopus)
287 Downloads (Pure)

Functional Interaction between Angiotensin II Receptor Type 1 and Chemokine (C-C motif) Receptor 2 with Implications for Chronic Kidney Disease

Ayoub, M., Zhang, Y., Kelly, R. S., See, E., Johnstone, E., Mccall, E. A., Williams, J. H., Kelly, D. J. & Pfleger, K., 25 Mar 2015, In : PLoS One. 10, 3, 22 p., e0119803.

Research output: Contribution to journalArticle

Open Access
File
CCR Receptors
Angiotensin Type 1 Receptor
angiotensin II
G-Protein-Coupled Receptors
kidney diseases
2014
19 Citations (Scopus)

Biophysical detection of diversity and bias in GPCR function

Jaeger, W., Armstrong, S., Hill, S. J. & Pfleger, K., 2014, In : Frontiers in Endocrinology. 5, MAR, p. 1-11

Research output: Contribution to journalReview article

Open Access
G-Protein-Coupled Receptors
Energy Transfer
Ligands
Arrestins
Fluorescence Resonance Energy Transfer
7 Citations (Scopus)

Homogeneous time-resolved fluorescence-based assay to monitor extracellular signal-regulated kinase signaling in a high-throughput format

Ayoub, M., Trebaux, J., Vallaghe, J., Charrier-Savournin, F., Al-Hosaini, K., Gonzalez Moya, A., Pin, J-P., Pfleger, K. & Trinquet, E., 2014, In : Frontiers in Endocrinology. 5, p. 1-11

Research output: Contribution to journalArticle

Open Access
12 Citations (Scopus)

Molecular determinants of orexin receptor-arrestin-ubiquitin complex formation

Jaeger, W., Seeber, R., Eidne, K. & Pfleger, K., Jan 2014, In : British Journal of Pharmacology. 171, 2, p. 364-374

Research output: Contribution to journalArticle

Open Access
2013
15 Citations (Scopus)

AIGaN/GaN-based biosensor for label-free detection of biological activity

Podolska, A., Hool, L., Pfleger, K., Mishra, U. K., Parish, G. & Nener, B., 2013, In : SENSORS AND ACTUATORS B-CHEMICAL. 177, p. 577-582

Research output: Contribution to journalArticle

19 Citations (Scopus)

Characterization of Three Vasopressin Receptor 2 Variants: An Apparent Polymorphism (V266A) and Two Loss-of-Function Mutations (R181C and M311V)

Armstrong, S., Seeber, R., Ayoub, M., Feldman, B. J. & Pfleger, K., 2013, In : PLoS One. 8, 6, p. 9pp e65885.

Research output: Contribution to journalArticle

Open Access
31 Citations (Scopus)

Identification and profiling of CXCR3-CXCR4 chemokine receptor heteromer complexes

Watts, A. O., Van Lipzig, M. M. H., Jaeger, W., Seeber, R., Van Zwam, M., Vinet, J., Van Der Lee, M. M. C., Siderius, M. H., Zaman, G. J. R., Boddeke, H. W. G. M., Smit, M. J., Pfleger, K., Leurs, R. & Vischer, H. F., 2013, In : British Journal of Pharmacology. 168, 7, p. 1662-1674

Research output: Contribution to journalArticle

CXC Chemokines
Chemokine Receptors
CC Chemokines
Chemokines
CCR2 Receptors
9 Citations (Scopus)
Open Access
19 Citations (Scopus)

Quantitative Förster resonance energy transfer efficiency measurements using simultaneous spectral unmixing of excitation and emission spectra

Mustafa, S., Hannagan, J., Rigby, P., Pfleger, K. & Corry, B., 2013, In : Journal of Biomedical Optics. 18, 2, p. 026024-1 - 026024-14

Research output: Contribution to journalArticle

Open Access
2012
17 Citations (Scopus)

Biocompatibility of semiconducting AIGaN/GaN material with living cells

Podolska, A., Tham, S., Hart, R. D., Seeber, R., Kocan, M., Kocan, M., Mishra, U. K., Pfleger, K., Parish, G. & Nener, B., 2012, In : SENSORS AND ACTUATORS B-CHEMICAL. 169, p. 401-406

Research output: Contribution to journalArticle

29 Citations (Scopus)

Deleterious GRM1 Mutations in Schizophrenia

Ayoub, M., Angelicheva, D., Vile, D., Chandler, D., Morar, B., Cavanaugh, J. A., Visscher, P. M., Jablensky, A., Pfleger, K. & Kalaydjieva, L., 2012, In : PLoS One. 7, p. 8pp e32849.

Research output: Contribution to journalArticle

Open Access

Detection of biological reactions by AlGaN/GaN biosensor

Podolska, A., Seeber, R., Mishra, U., Pfleger, K., Parish, G. & Nener, B., 2012, COMMAD 2012 PROCEEDINGS. United States: IEEE, Institute of Electrical and Electronics Engineers, Vol. 1. p. 75-76

Research output: Chapter in Book/Conference paperConference paper

35 Citations (Scopus)
36 Citations (Scopus)

Identification and profiling of novel α 1A-adrenoceptor-CXC chemokine receptor 2 heteromer

Mustafa, S., See, E., Seeber, R., Armstrong, S. P., White, C. W., Ventura, S., Ayoub, M. & Pfleger, K., 2012, In : Journal of Biological Chemistry. 287, 16, p. 12952-12965

Research output: Contribution to journalArticle

Open Access
11 Citations (Scopus)

mGlu5 receptor functional interactions and addiction

Brown, R. M., Mustafa, S., Ayoub, M., Dodd, P. R., Pfleger, K. & Lawrence, A. J., 2012, In : Frontiers in Pharmacology. 3, p. 1-9 84.

Research output: Contribution to journalReview article

Open Access

Nano-scale investigation of cell/semiconductor interface

Podolska, A., Tham, S., Hart, R. D., Seeber, R., Pfleger, K., Parish, G. & Nener, B., 2012, APMC10 / ICONN2012 / ACMM22 CONFERENCE PROCEEDINGS. Australia: The University of Western Australia, Vol. 1. p. 703-1 - 703-3

Research output: Chapter in Book/Conference paperConference paper

10 Citations (Scopus)

Receptor-Heteromer investigationtechnology and its application using BRET

Johnstone, E. & Pfleger, K. D. G., 2012, In : Frontiers in Endocrinology. 3, AUG, Article 101.

Research output: Contribution to journalReview article

Open Access
Energy Transfer
Pharmacology
Technology
Dermatoglyphics
Pathology

Enhanced BRET technology for the monitoring of agonist-induced and agonist-independent interactions between GPCRs and β-arrestins

Kocan, M., Dalrymple, M., Seeber, R., Feldman, B. J. & Pfleger, K., 2011, In : Frontiers in Endocrinology. 1, p. 1-9 12.

Research output: Contribution to journalArticle

14 Citations (Scopus)
Open Access
35 Citations (Scopus)

Heteromerization of angiotensin receptors changes trafficking and arrestin recruitment profiles

Porrello, E. R., Pfleger, K., Seeber, R., Qian, H., Oro, C., Abogadie, F., Delbridge, L. M. D. & Thomas, W. G., 2011, In : Cellular Signalling. 23, p. 1767-1776

Research output: Contribution to journalArticle

20 Citations (Scopus)

Study of GPCR-Protein Interactions by BRET

Kocan, M. & Pfleger, K., 2011, Receptor Signal Transduction Protocols. Willars, G. B. & Challiss, R. A. J. (eds.). Third Edition ed. New York: Humana Press, Vol. 746. p. 357-371 (Methods in Molecular Biology).

Research output: Chapter in Book/Conference paperChapter

20 Citations (Scopus)

Temporal Profiling of Orexin Receptor-Arrestin-Ubiquitin Complexes Reveals Differences between Receptor Subtypes

Dalrymple, M., Jaeger, W., Eidne, K. & Pfleger, K., 2011, In : Journal of Biological Chemistry. 286, p. 16726-16733

Research output: Contribution to journalArticle

Open Access
2010
47 Citations (Scopus)

Differential association modes of the thrombin receptor PAR1 with Gαi1, Gα12, and β-arrestin 1

Ayoub, M., Trinquet, E., Pfleger, K. & Pin, J-P., 2010, In : FASEB Journal. 24, p. 3522-3535

Research output: Contribution to journalArticle

Ion sensitive AlGaN/GaN heterostructures for cell-based biosensor development

Podolska, A., Dunnage, S., Umana Membreno, G. A., Seeber, R., Fehlberg, T., Keller, S., Mishra, U., Pfleger, K., Nener, B. & Parish, G., 2010, 2010 Conference on Optoelectronic and Microelectronic Materials and Devices COMMAD 2010 Proceedings. Tan, H. H. (ed.). Canberra, ACT, Australia ed. United States of America: IEEE, Institute of Electrical and Electronics Engineers, Vol. 1. p. 137-138

Research output: Chapter in Book/Conference paperConference paper

4 Citations (Scopus)

Monitoring GPCR-Protein Complexes Using Bioluminescence Resonance Energy Transfer

Jaeger, W. C., Pfleger, K. & Eidne, K., 2010, G Protein-Coupled Receptors. Poyner, D. R. & Wheatley, M. (eds.). United Kingdom: John Wiley & Sons, p. 111-132

Research output: Chapter in Book/Conference paperChapter

73 Citations (Scopus)
14 Citations (Scopus)

Uncovering GPCR heteromer-biased ligands

Mustafa, S., Ayoub, M. & Pfleger, K., 2010, In : Drug Discovery Today. 7, p. 77-85

Research output: Contribution to journalArticle

2009
33 Citations (Scopus)

Agonist-independent interactions between beta-arrestins and mutant vasopressin type II receptors associated with nephrogenic syndrome of inappropriate antidiuresis

Kocan, M., See, E., Sampaio, N., Eidne, K., Feldman, B. J. & Pfleger, K., 2009, In : Molecular Endocrinology. 23, 4, p. 559-571

Research output: Contribution to journalArticle

Vasopressin Receptors
Hyponatremia
Codon
Mutation
Arginine
8 Citations (Scopus)

Analysis of Protein-Protein Interactions Using Bioluminescence Resonance Energy Transfer

Pfleger, K., 2009, Bioluminescence Methods and Protocols. Rich, Douillet, P. B. & C. (eds.). United States: Humana Press, p. 173-183

Research output: Chapter in Book/Conference paperChapter

256 Citations (Scopus)

Building a new conceptual framework for receptor heteromers

Ferré, S., Baler, R., Bouvier, M., Caron, M. G., Devi, L. A., Fuxw, K., Durroux, T., Javitch, J. A., George, S. R., Lohse, M. J., Mackie, K., Milligan, G., Pfleger, K., Pin, J. P., Volkow, N. D., Waldhoer, M., Woods, A. S. & Franco, R., 2009, In : Nature Chemical Biology. 5, 3, p. 131-134

Research output: Contribution to journalArticle