Photo of Elaine Wong

Elaine Wong

Dr

  • The University of Western Australia (M518), 35 Stirling Highway,

    6009 Perth

    Australia

  • 361 Citations
  • 10 h-Index
20022019
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Personal profile

Biography

Elaine Wong is the Senior Research Fellow at Ear Science Institute Australia and School of Pharmacy & Biomedical Sciences in Curtin University. She has an appointment as Adjunct Senior Research Fellow at the Ear Sciences Centre, Division of Surgery, Faculty of Health and Medical Sciences, The University of Western Australia. 

She graduated from the University of Hong Kong in 1999, and she completed a Mphil in 2002 and a PhD in 2006 at the University of Hong Kong. Dr Wong was a visiting scholar in inner ear neurobiology at the University of Iowa in 2008.  She underwent her second post-doctoral training in inner ear developmental and regenerative biology in Mount Sinai School of Medicine in New York in 2010-2013. Elaine worked as a Research Assistant Professor in the School of Biomedical Sciences, HKU in 2013-2017.

Since joining Ear Science in July 2017 she has been involved in inner ear organoids from pluripotent stem cells for patient-specific ear development model, inner ear developmental biology, human and mouse molecular genetics research in ear and hearing therapy. Her research interest includes understanding the genetic and molecular mechanisms in human deafness and regeneration of hair cells in mammalian inner ear; and understanding regulatory networks controlling the age-related hearing loss, Human Branchio-oto-renal syndrome and Usher syndrome.

She is currently working on the Human pluripotent stem cell differentiation to inner ear tissue for Usher syndrome model. This project is funded by the Stan & Jean Perron Charitable Foundation and started on 2018-2021 to support “the Stan & Jean Perron cure hearing loss team to perform Human pluripotent stem cell differentiation to inner ear tissue for Usher syndrome model’.

Her laboratory also focuses on studying the roles of the transcription factors in the regeneration of hair cells in inner ears. The project is currently supported by The Garnett Passe and Rodney Williams Memorial Foundation Australia and started on April 2018. The long-term goal is to develop therapies for prevention, repair and regeneration in human deafness. 

Dr Wong’s research has attracted over $1.5 million in research funding from Australian, Hong Kong and China government and industry. She has developed active collaboration with various institutions including Lions Eye Institute, Perth; University of Newcastle in Australia; Peking Union Medical College and Southern University of Science and Technology in China; The University of Hong Kong.

Research Interest

Her research focuses on understanding the potential of hair cell regeneration to address hearing impairment in Usher syndrome and develop a new therapeutic treatment for deafness in humans.

(1) Using Usher patient-derived induced pluripotent stem cells to generate human inner ear and retinal organoids and study the underlying molecular and cellular mechanisms in hearing loss for inner ear cell therapies by using CRISPR/Cas9 gene editing techniques;

(2) Sensory hair cells, supporting cells and neuronal differentiation by using human cell and animal models for studying regenerative cell biology in the inner ears;

(3) Genetic, molecular and cellular causes of vestibular balancing and hearing loss and their treatment.

Using human and mouse genetic approaches, including conditional gene targeting and inducible expression systems to elucidate key molecular pathways involved in different aspects of inner ear development.

Techniques involve human induced pluripotent stem cell, inner ear organoid culture, multiphoton confocal live cell imaging and confocal microscopy, gene manipulation in mouse embryos and cochlea tissues by CRISPR/Cas9 to generate loss-of-function system or overexpressing the target gene for gain-of-function system, flow cytometry, high resolution electron microscopy SEM, ultrastructural localization of the proteins involved in the stereocilia bundle at the top of sensory hair cells in the organ of Corti, quantitative PCR, coimmunoprecipitation CoIP, single cell RNA sequencing, ChIP sequencing and bioinformatics analyses.

Email address:

elaine.wong@earscience.org.au

Education/Academic qualification

Developmental Biology, Doctor of Philosophy PhD

Molecular Biology, Master of Philosophy MPhil

Biotechnology and Biology, Bachelor of Science

External positions

Senior Research Fellow, Curtin University of Technology

1 Jul 2017 → …

Senior Research Fellow, Ear Sciences Institute Australia

1 Jul 2017 → …

Keywords

  • Biotechnology
  • Drug Discovery
  • Stem cell and cell therapy
  • Genetic therapies
  • Hearing and deafness
  • Developmental neuroscience
  • Drug delivery

Fingerprint Dive into the research topics where Elaine Wong is active. These topic labels come from the works of this person. Together they form a unique fingerprint.

  • 5 Similar Profiles
Cells Chemical Compounds
Proteins Medicine & Life Sciences
Inner Ear Medicine & Life Sciences
Phosphoric Monoester Hydrolases Medicine & Life Sciences
Genes Medicine & Life Sciences
Mutation Medicine & Life Sciences
Auditory Hair Cells Medicine & Life Sciences
Testis Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Research Output 2002 2019

  • 361 Citations
  • 10 h-Index
  • 13 Article
  • 1 Review article

Generation of two induced pluripotent stem cell lines from a patient with compound heterozygous mutations in the USH2A gene

McLenachan, S., Wong, E. Y. M., Zhang, X., Leith, F., Moon, S. Y., Zhang, D., Chen, S. C., Thompson, J. A., McLaren, T., Lamey, T., De Roach, J. N., Atlas, M. D., Dilley, R. J. & Chen, F. K., 1 Apr 2019, In : Stem Cell Research. 36, 101420.

Research output: Contribution to journalArticle

Open Access
Usher Syndromes
Induced Pluripotent Stem Cells
Pluripotent Stem Cells
Endoderm
Mesoderm
2 Citations (Scopus)

Localization of TMC1 and LHFPL5 in auditory hair cells in neonatal and adult mice

Li, X., Yu, X., Chen, X., Liu, Z., Wang, G., Li, C., Wong, E. Y. M., Sham, M. H., Tang, J., He, J., Xiong, W., Liu, Z. & Huang, P., Jun 2019, In : FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 33, 6, p. 6838-6851 14 p.

Research output: Contribution to journalArticle

Auditory Hair Cells
Lipoma
Fusion reactions
Cells
Stereocilia
5 Citations (Scopus)

An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis

Zhang, H., Wang, L., Wong, E. Y. M., Tsang, S. L., Xu, P-X., Lendahl, U. & Sham, M. H., 15 Nov 2017, In : eLife. 6, e30126.

Research output: Contribution to journalArticle

Open Access
Branchial Region
Arches
Morphogenesis
Phosphoprotein Phosphatases
Sensory Receptor Cells
12 Citations (Scopus)

EYA1's Conformation Specificity in Dephosphorylating Phosphothreonine in Myc and Its Activity on Myc Stabilization in Breast Cancer

Li, J., Rodriguez, Y., Cheng, C., Zeng, L., Wong, E. Y. M., Xu, C. Y., Zhou, M-M. & Xu, P-X., Jan 2017, In : Molecular and Cellular Biology. 37, 1, 18 p., e00499-16.

Research output: Contribution to journalArticle

Phosphothreonine
Breast Neoplasms
Phosphoric Monoester Hydrolases
Protein Stability
Phosphoprotein Phosphatases
7 Citations (Scopus)

A Novel ENU-Induced Mutation in Myo6 Causes Vestibular Dysfunction and Deafness

Wong, E. Y. M., Xu, C. Y., Brahmachary, M. & Xu, P-X., 2016, In : PLoS One. 11, 5, p. e0154984

Research output: Contribution to journalArticle

Open Access
N-ethyl-N-nitrosourea
Ethylnitrosourea
deafness
Deafness
mutagenesis