Single-molecule imaging and correlative microscopy
With A/Prof Haibo Jiang (HKU)
We are developing methods to amplify signals in cells and detect individual molecules can help us pinpoint the location of therapeutic molecules and track their journey through cells. We are also using expansion microscopy, a technique in which tissue samples are embedded in a swellable hydrogel and expanded in size before looking under the microscope. These experiments will help us understand the trafficking and distribution of the latest RNA-based therapeutics.

Macromolecular transfection agent design
With Prof K Swaminathan Iyer and Dr Marck Norret (University of Western Australia) and A/Prof Pilar Blancafort (Perkins Institute of Medical Research)
Gene therapies help to reprogramme cells, correcting errors in gene expression that cause disease. We are designing macromolecules that help to introduce nucleic acids, proteins, and gene editing platforms into living cell cultures. We have developed this technology to allowing barcoding and multiplexing of single-cell sequencing samples. Our platform could lead to the translation of novel gene therapies as well as tools for scientists to investigate the roles of specific genes.

Multiplex single-cell sequencing in breast cancer models
With A/Prof Alka Saxena (Genomics WA)
We are examining metastasis and drug resistance in breast cancer using models of epithelial–mesenchymal transition in cells. Using the latest single-cell sequencing techniques, we are investigating how cancer cells change and acquire invasive properties. Our work may help uncover new ways to improve the response to anticancer therapies and prevent tumour recurrence in breast cancer.

Designing hydrogels for delivery of therapies
With A/Prof Joost Lesterhuis (Telethon Kids Institute) and Dr Rabab Rashwan (Lions Eye Institute)
I have developed hydrogels for delivery of immunotherapies in sarcoma, which are now being tested in domestic dogs that have had tumours removed by a veterinary oncologist. The gels help to prevent recurrence by activating the immune system to help eradicate remaining cancer cells. We are also developing hydrogels to deliver a sustained dose of drug to the eye to combat retinitis pigmentosa—a genetic, blinding retinal disorder.

Liposomal formulation of therapeutic oligonucleotides
With A/Prof Rakesh Veedu (Murdoch University) and Prof Peter Leedman (Perkins Institute of Medical Research)
I am collaborating with research groups to help them encapsulate therapeutic oligos in lipid nanoparticles for successful delivery of therapies against liver cancer and other diseases. Encapsulation in lipid nanoparticles helps to protect the oligo against degradation, and ensures effective delivery to the site of action.

Non-canonical nucleic acid structures in gene regulation and disease
With Dr Nicole Smith (University of Western Australia)
We are investigating the role of unusual DNA and RNA structures (G-quadruplexes and i-motifs) in gene regulation, and how these structures can be harnessed for therapy.

STEM outreach: Chair of Outreach committee, ARC Training Centre in Biomedical Analysis, offering activities to UWA visitors including strong Indigenous representation, exploring nanotechnology and 3D visualisation.

Nanotechnology demonstrations in high schools: I currently co-ordinate an exciting programme of nanotechnology demos in WA high schools. Please get in touch if you would like us to come to you!