Alice Vrielink

Professor, BSc MSc Calg., PhD Lond.

  • The University of Western Australia (M310), 35 Stirling Highway, Room 431, Bayliss Building, Perth campus

    6009 Crawley

    Australia

  • 1629 Citations
  • 20 h-Index
20002020
If you made any changes in Pure these will be visible here soon.

Personal profile

Biography

Professor Alice Vrielink obtained her Bachelor of Science in Chemistry and Masters of Science in Physical Chemistry at the University of Calgary in Canada. She then completed a PhD in Physics at the University of London and a Diploma in Crystallography from Imperial College of Science and Technology, London. From 1994 – 2001 Prof. Vrielink was an Assistant and Associate Professor at McGill University in Canada. She then continued her research as a Research Professor at the University of California, Santa Cruz. In 2007 she joined the faculty at UWA as Professor of Structural Biology

Roles and responsibilities

Head of Discipline (Biochemistry & Molecular Biology)

Funding overview

Raine Medical Research Foundation Priming Grant
National Health and Medical Research Council Project Grant
Australian Research Council Large Equipment and Infrastructure Fund Grants

Cancer Council of Western Australia

iCare Dust Diseases Care

Current projects

Collaborators
Prof Grant Hartzog, University of California, Santa Cruz
Dr. Keith Stubbs, Chemistry, UWA; Prof Barry Marshall, Microbiology, UWA
Prof Charlene Kahler, Microbiology, UWA
Dr. Flora Meilleur, Oak Ridge National Laboratory, USA

Dr. Willem Lesterhuis, School of Biomedical Sciences, UWA,

Dr. Matthew Piggot, Chemistry, UWA

Teaching overview

BIOC3002 (Unit Co-ordinator) Structural and Functional Biochemistry
BIOC3001 - Molecular Biology
BIOC2002 - Biochemical Regulation of Cell Function

MSCI4006 - Advanced Techniques in Molecular Science

Research

Dr Vrielink uses protein crystallography to study the 3D structures of macromolecules. In particular, she is interested in studying enzymes involved in redox catalysis, proteins involved in bacterial pathogenesis and proteins involved in the process of transcription elongation. Understanding the structures of these macromolecules provides important insight into their molecular and biological functions.

In collaboration with Prof Charlene Kahler (Microbiology and Immunology), Dr. Keith Stubbs (Chemistry) and Prof Martin Scanlon (Monash University) Dr Vrielink is working in the area of endotoxin biosynthesis in Neisseria meningitidis, the causative agents of meningitis. Endotoxin molecules on these bacteria are decorated with multiple phosphoethanolamine (PEA) groups, the synthesis of which is catalyzed in part by the enzyme LptA. Inactivation of LptA increases bacterial sensitivity to defensins, suggesting that the PEA groups prevent defensin attachment to the bacterial surface. In an effort to better characterize this enzyme Dr. Vrielink has determined the three dimensional structure of the LptA enzyme. These structural studies are being correlated with functional studies carried out in Prof Kahler’s laboratory. Together with Dr. Stubbs and Prof Scanlon fragment screening and inhibitor design studies are being undertaken as steps towards development of novel antimicrobial therapeutics to treat multidrug resistance in bacteria.

In collaboration with Dr. Grant Hartzog (University of California Santa Cruz) work is being carried out aimed at understanding the function of the ubiquitous transcription elongation factor Spt4/5 from yeast. In specific we are characterizing the RNA binding specificity of this protein and the role of the protein in transcription.

In collaboration with Dr. Flora Meilleur (Oak Ridge National Laboratory) neutron crystallography studies are being undertaken on the flavoenzyme, cholesterol oxidase. These studies are directed at understanding the redox activity of the enzyme through characterizing the positions of hydrogen atoms within the protein during the catalytic cycle.

Keywords

  • Protein crystallography
  • Enzyme mechanism
  • Flavoenzymes
  • Multifunctional enzymes and substrate channeling
  • Membrane protein structure and function
  • Bacterial virulence factors
  • Transcription factors
  • Protein-nucleic acid interactions
  • Protein structure by X-ray crystallography

Fingerprint Fingerprint is based on mining the text of the person's scientific documents to create an index of weighted terms, which defines the key subjects of each individual researcher.

  • 2 Similar Profiles
Cholesterol Oxidase Medicine & Life Sciences
Enzymes Medicine & Life Sciences
Catalytic Domain Medicine & Life Sciences
Proteins Medicine & Life Sciences
Flavin-Adenine Dinucleotide Medicine & Life Sciences
Lipid A Medicine & Life Sciences
Hydrogen Medicine & Life Sciences
Oxidoreductases Medicine & Life Sciences

Network Recent external collaboration on country level. Dive into details by clicking on the dots.

Projects 2007 2020

Research Output 2000 2018

  • 1629 Citations
  • 20 h-Index
  • 48 Article
  • 3 Review article
  • 2 Chapter
  • 1 Literature review
Open Access
Lipid A
Drug Delivery Systems
Enzymes
Endotoxins
Antimicrobial Cationic Peptides

The first transcriptomes from field-collected individual whiteflies (Bemisia tabaci, Hemiptera: Aleyrodidae)

Sseruwagi, P., Wainaina, J. M., Ndunguru, J. C., Tumuhimbise, R., Tairo, F., Guo, J., Vrielink, A., Blythe, A. J., KINENE, T., de Marchi, B. R., Kehoe, M. A. & Boykin, L. M., 13 Feb 2018, In : Gates Open Research. 1, p. 16

Research output: Contribution to journalArticle

Open Access
Bemisia tabaci
Aleyrodidae
transcriptome
Hemiptera
Sub-Saharan Africa
2 Citations

Acquisition of the capsule locus by horizontal gene transfer in Neisseria meningitidis is often accompanied by the loss of UDP-GalNAc synthesis

Bartley, S. N., Mowlaboccus, S., Mullally, C. A., Stubbs, K. A., Vrielink, A., Maiden, M. C. J., Harrison, O. B., Perkins, T. T. & Kahler, C. M., 14 Mar 2017, In : Scientific Reports. 7, 14 p., 44442

Research output: Contribution to journalArticle

Open Access
Horizontal Gene Transfer
Uridine Diphosphate
Neisseria meningitidis
Capsules
Islands
2 Citations

An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase

Golden, E., Yu, L. J., Meilleur, F., Blakeley, M. P., Duff, A. P., Karton, A. & Vrielink, A., 18 Jan 2017, In : Scientific Reports. 7, p. 12 12 p., 40517

Research output: Contribution to journalArticle

Open Access
Cholesterol Oxidase
Hydrogen
Oxidation-Reduction
Oxidoreductases
Nitrogen
1 Citations

Computational insights for the hydride transfer and distinctive roles of key residues in cholesterol oxidase

Yu, L., Golden, E. A., Chen, N., Zhao, Y., Vrielink, A. & Karton, A., 8 Dec 2017, In : Scientific Reports. 7, 13 p., 17265

Research output: Contribution to journalArticle

Open Access
Cholesterol Oxidase
Choline
Methanol
Glucose
Flavin-Adenine Dinucleotide

Courses

BIOC2002

Alice Vrielink

30/06/15 → …

Teaching unit (UWA): Course

BIOC3001

Alice Vrielink

24/02/14 → …

Teaching unit (UWA): Course

BIOC3002

Alice Vrielink

23/01/07 → …

Teaching unit (UWA): Course

MSCI4006

Alice Vrielink

27/01/15 → …

Teaching unit (UWA): Course