Thermo Scientific Orbitrap ID-X Tribrid Mass Spectrometer System

The ID-X system consists of a Vanquish UPLC system coupled to a tribrid mass spectrometer furnished with an atmospheric pressure ion source. The mass spectrometer combines a quadrupole, ion trap and high-resolution, accurate-mass (HRAM) Orbitrap mass analysers for acquiring multi-stage mass spectrometry (MSn) data. Collision-induced dissociation (CID) and high-energy collisional dissociation (HCD) fragmentation techniques are available at any stage of MSn. This unique architecture allows for multiple data independent and data dependant acquisition modes to be carried out using the Thermo Scientific Acquire X data acquisition tool. Thermo Scientific Compound Discoverer data analysis software streamlines unknown identification, determination of differences and elucidation of biological pathways.

Techniques

A high-throughput, untargeted metabolomics method focused on the global detection of small molecules in biological samples is currently under development.

Specifications

•Mass range: m/z 50-2,000

•Orbitrap mass analyser resolution: 7,500-500,000 (FWHM) at m/z 200

•Mass accuracy:

   <3 ppm RMS using external calibration

   <1 ppm RMS using internal calibration

•MS scan power: MSn, for n = 1-10

To cite this equipment/component: Centre for Microscopy, Characterisation & Analysis. (2025). Thermo Scientific Orbitrap ID-X Tribrid Mass Spectrometer System. The University of Western Australia. https://doi.org/10.26182/S420-EH53