Nikon STORM (inverted)

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Description

    Localisation imaging is the final super resolution microscope platform in our suite. Imaging with this modality exploits the photophysical behaviour of fluorophores to Our N-STORM system is built on an inverted Ti-E microscope, fitted with four solid state lasers (405nm, 488nm, 561nm and 640nm) and a Hamamatsu OCRA FLASH 4 sCMOS camera, for high speed acquisition. C-STORM imaging can be performed on this platform, as well as the novel N-STORM imaging modality, where tandem dye pairs are used conjugated to samples utilising the high performance of Alexafluor 647 fluorescent dye.

    The system is also equipped with Tokai Hit stage top incubator for live cell imaging, and Perfect Focus System (PFS) to prevent drift.

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    platforms
    dyes
    microscopes
    solid state lasers
    acquisition
    cameras
    high speed
    cells

    Research technique

    • Stochastic Optical Reconstruction Microscopy (STORM)
    • Total Internal Reflection Microscopy (TIRF)
    • Live cell imaging