Nikon SIM (Ti2) (inverted)

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Description

    Housed on a state-of-the-art Ti2 microscope, our SIM platform can acquire images with a resolution down to 100nm. Various objectives are available for this system, including 100x oil immersion with motorized correction collar, and 60x WI objective, specialized for live cell imaging. It is a four colour platform (405nm, 488nm, 561nm and 640nm solid state lasers), where images are acquired on an Andor iXon 897 EMCCD camera.

    In addition to this, the system is capable of imaging in either 2D SIM, 3D SIM, 3D stack SIM or TIRF SIM mode for fixed or live cells (Tokai Hit stage top incubation system). Stability of the field of view over the imaging period is enabled by the Piezo stage and Perfect Focus System (PFS).

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    Imaging techniques
    Solid state lasers
    Microscopes
    Cameras
    Color
    Oils

    Research technique

    • 3D volumetric imaging
    • Live cell imaging