Nikon A1Si confocal microscope

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Equipments Details

    Description

    Nikon Ti-E inverted motorised microscope with Nikon A1Si spectral detector confocal system running NIS-C Elements software.

    The system is capable of simultaneous or sequential image collection using four photomultiplier fluorescence detectors, a 32-channel spectral detector and a transmission detector. Long-term time-lapse imaging is possible using the Tokai Hit stage top incubation system.

    Two colour (488nm and 561nm) total internal reflection fluorescence (TIRF) microscopy is integrated into the microscope and high-speed image capture is available using the Andor 885 EMCCD camera, all of which is controlled by the NIS Elements software.

    Research technique

    • Confocal microscopy
    • Multipoint imaging
    • Spectral detection and unmixing

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