The Leica is an inverted microscope with eight laser and multi-photon excitations. The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached.Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.
To cite this equipment/component: Centre for Microscopy, Characterisation & Analysis. (2018). Leica TCS SP2 multiphoton confocal microscope. University of Western Australia. https://doi.org/10.26182/CPFD-F918
Name | Decommissioned |
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Decommission date | 1/01/19 |
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- Confocal microscopy
- Multiphoton microscopy
- Fluorescence recovery after photobleaching (FRAP)
- Fluorescence resonance energy transfer (FRET)
- Secondary harmonic generation (SHG)
- Fluorescence loss in photobleaching (FLIP)
- Fluorescence Correlation Spectroscopy (FCS)