The Leica is an inverted microscope with eight laser and multi-photon excitations. The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached. Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.
Fluorescence Resonance Energy Transfer