Leica TCS SP2 multiphoton confocal microscope

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Description

    The Leica is an inverted microscope with eight laser and multi-photon excitations. The system is capable of simultaneous image collection, quantitative confocal image analysis, 3D reconstruction and most multiphoton microscopy techniques. A separate Becker & Hickle Fluorescence Loss Imaging Microscopy (FLIM) analysis system is also attached. Software includes 3D reconstruction with rendering, multi-colour analysis, fluorescence recovery after photobleaching (FRAP) analysis, fluorescence resonance energy transfer (FRET) analysis, animation, stereo imaging, single layer projection and quantitative analysis, time-lapse collection, ratio quantification and co-localisation analysis of two or three probes.

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    Microscopic examination
    Microscopes
    Fluorescence
    Imaging techniques
    Photobleaching
    Animation
    Image analysis
    Photons
    Color
    Recovery
    Lasers
    Chemical analysis
    Fluorescence Resonance Energy Transfer

    Research technique

    • Confocal microscopy
    • Multiphoton microscopy
    • Fluorescence recovery after photobleaching (FRAP)
    • Fluorescence resonance energy transfer (FRET)
    • Secondary harmonic generation (SHG)
    • Fluorescence loss in photobleaching (FLIP)
    • Fluorescence Correlation Spectroscopy (FCS)