BD SORP Fortessa

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Description

    This high-end instrument allows polychromatic flow cytometry analysis up to 18 different colours (17 simultaneously). It is equipped with a forward scatter PMT for analysis of submicron particles, and a high throughput sampler (HTS) allows automated analysis of 96 or 384 samples in plates, each with a volume as low as 22┬ÁL.

    Analysis rates of up to 35,000 events per second and standard analysis time for a 96 well plate of 44 minutes in (approximately 15 minutes in high throughput mode).

    Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential. Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean- fluorescence intensity within the sample.

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    Flow cytometry
    Population statistics
    Throughput
    Plant DNA
    Fluorescence
    Cells
    RNA
    Apoptosis
    Cytokines
    Calcium
    Color
    Membranes
    DNA