A high throughput sampler (HTS) allows automated analysis of 96 or 384 samples, each with a volume as low as 3µL.
Analysis rates of up to 10,000 events per second and minimum analysis time for a 96 well plate is 15 minutes.
Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential. Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.
To cite this equipment/component: Centre for Microscopy, Characterisation & Analysis. (2018). BD Canto II. University of Western Australia. https://doi.org/10.26182/2BYJ-8S04
- Flow Cytometry Cell Profiling