BD Canto II

    Facility/equipment: Equipment

    • Location

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Description

    A high throughput sampler (HTS) allows automated analysis of 96 or 384 samples, each with a volume as low as 3┬ÁL.

    Analysis rates of up to 10,000 events per second and minimum analysis time for a 96 well plate is 15 minutes.

    Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential. Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.

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    Plant DNA
    Immunophenotyping
    Respiratory Burst
    Mitochondrial Membrane Potential
    Ploidies
    Population Characteristics
    Cell Cycle
    Flow Cytometry
    Fluorescence
    RNA
    Apoptosis
    Cytokines
    Calcium
    DNA