BD Canto II

    Facility/equipment: Equipment

    • LocationShow on map

      CMCA@Perkins, (M519), Level 3, Harry Perkins Institute of Medical Research, 6 Verdun St, Perth WA 6009

      Australia

    Equipments Details

    Description

    A high throughput sampler (HTS) allows automated analysis of 96 or 384 samples, each with a volume as low as 3µL.

    Analysis rates of up to 10,000 events per second and minimum analysis time for a 96 well plate is 15 minutes.

    Analyses include immunophenotyping, viability, DNA content, cell cycle, plant ploidy, DNA/RNA ratios, apoptosis measurements, cytokine expression, intracellular calcium, oxidative burst, intracellular pH, phagocytic activity and mitochondrial membrane potential. Flow cytometry analysis generates bivariate dot-plots and histograms which can be used to calculate population statistics and changes in mean-fluorescence intensity within the sample.

    To cite this equipment/component: Centre for Microscopy, Characterisation & Analysis. (2018). BD Canto II. University of Western Australia. https://doi.org/10.26182/2BYJ-8S04

    Research technique

    • Flow Cytometry Cell Profiling

    Fingerprint

    Explore the research areas in which this equipment has been used. These labels are generated based on the related outputs. Together they form a unique fingerprint.