Single Nucleus RNA Sequencing of Pre-Malignant Liver Reveals Disease-Associated Hepatocyte State with HCC Prognostic Potential

  • Rodrigo Carlessi (Creator)
  • Elena Denisenko (Creator)
  • Ebru Boslem (Creator)
  • Julia Köhn-Gaone (Creator)
  • Nathan Main (Creator)
  • N. Dianah B. Abu Bakar (Creator)
  • Gayatri Shirolkar (Curtin University) (Creator)
  • Matt Jones (Creator)
  • Daniel Poppe (Contributor)
  • Benjamin J. Dwyer (Creator)
  • Connie Jackaman (Creator)
  • Christian Tjiam (Curtin University, The Kids Research Institute Australia (Telethon Kids Institute) (Creator)
  • Ryan Lister (Creator)
  • Michael Karin (Creator)
  • Jonathan A. Fallowfield (Creator)
  • Timothy J. Kendall (Creator)
  • Stuart J. Forbes (Creator)
  • John K. Olynyk (Creator)
  • George Yeoh (Creator)
  • Alistair Forrest (Creator)
  • Grant Ramm (Creator)
  • Mark A. Febbraio (Creator)
  • Nina Tirnitz-Parker (Curtin University, Harry Perkins Institute of Medical Research) (Creator)

Dataset

Description

Expression profiling by high throughput sequencing.

To identify and characterize cell states associated with the chronically injured pre-malignant liver, we employed a droplet-based (10x chromium) single nucleus transcriptomics approach. Hepatic nuclei were isolated and profiled from (a) healthy mice fed normal chow, (b) mice subjected to a choline-deficient, ethionine-supplemented (CDE) diet, and (c) mice provided with thioacetamide (TAA) in the drinking water. We obtained a total of 40,748 single nucleus transcriptomes (16,222 healthy; 14,507 CDE; and 10,019 TAA) from three mice per condition.
Date made available12 Apr 2022
PublisherGene Expression Omnibus (NCBI)

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