Data from: Parasite load and MHC diversity in undisturbed and agriculturally modified habitats of the ornate dragon lizard

  • Jacek Radwan (Creator)
  • Katarzyna Kuduk (Creator)
  • Esther Levy (Creator)
  • Natasha Lebas (Creator)
  • Wieslaw Babik (Creator)



MHC gene polymorphism is thought to be driven by host-parasite coevolution, but the evidence for an association between the selective pressure from parasites and the number of MHC alleles segregating in a population is scarce and inconsistent. Here, we characterized MHC class I polymorphism in a lizard whose habitat preferences (rock outcrops) lead to the formation of well-defined and stable populations. We investigated the association between the load of ticks, which were used as a proxy for the load of pathogens they transmit, and MHC class I polymorphism across populations in two types of habitat: undisturbed reserves and agricultural land. We hypothesized that the association would be positive across undisturbed reserve populations, but across fragmented agricultural land populations the relationship would be distorted by the loss of MHC variation due to drift. After controlling for habitat, MHC diversity was not associated with tick number, and the habitats did not differ in this respect. Neither did we detect a difference between habitats in the relationship between MHC and neutral diversity, which was positive across all populations. However, there was extensive variation in the number of MHC alleles per individual, and we found that tick number was positively associated with the average number of alleles carried by lizards across reserve populations, but not across populations from disturbed agricultural land. Our results thus indicate that local differences in selection from parasites may contribute to MHC copy number variation within species, but habitat degradation can distort this relationship.,MHC class I allelesCtenophorus ornatus MHC I putative functional allele sequences. Aligned fasta file.Ctenophorus_ornatus_MHC_I_putative_functional_alleles.fasIndividual tick count and number of MHC alllesCtenophorus ornatus Individual tick count and number of MHC allles. Information about habitat and population of origin is also included. Excel format.Ctenophorus_ornatus_tick_load_n_MHC_alleles.xlsxMHC and microsatellite genotypesMHC and microsatellite genotypes. MHC genotypes are in Structure-like dominant format (each allele trated as a separate locus). Microsatellite genotypes are in Structure-like one-line-per genotype format. Excel file.Ctenophorus_ornatus_MHC_msat_genotypes.xlsxCtenophorus_ornatus_expression_dataThis file contains results of MHC genotyping of 4 individuals using genomic DNA (gDNA) and cDNA synthesized from DNAse-treated RNA (cDNA); for one individual only cDNA data are available. Because in no gDNA/cDNA pair coverage in both samples was sufficient for rigorous genotyping (min 300 reads), we present raw read counts of putative true alleles (identified as such using the entire dataset). On the basis of the read counts in gDNA/cDNA samples we assign "expressed", "not_expressed" and in few cases "undetermined" expression status to those alleles.,
Date made available29 Oct 2014
PublisherDryad Digital Repository

Cite this