Assembly and comparison of two closely related Brassica napus genomes

  • Philipp Bayer (Creator)
  • Bhavna Hurgobin (University of Queensland) (Creator)
  • Agnieszka Golicz (Creator)
  • Chon-Kit Kenneth Chan (The University of Western Australia) (Creator)
  • Andy Yuan (Creator)
  • HueyTyng Lee (University of Queensland) (Creator)
  • Michael Renton (Creator)
  • Jinling Meng (Creator)
  • Ruiyuan Li (Creator)
  • Yan Long (Creator)
  • Jun Zou (Creator)
  • Ian Bancroft (Creator)
  • Boulos Chalhoub (Creator)
  • Graham J King (Creator)
  • Jacqueline Batley (Creator)
  • David Edwards (The University of Western Australia) (Creator)



Here we present the de novo assembly of the B. napus cultivar Tapidor and comparison with an improved assembly of the B. napus cultivar Darmor-bzh. Both cultivars were annotated using the same method to allow comparison of gene content. We identified genes unique to each cultivar and differentiate these from artefacts due to variation in the assembly and annotation. We demonstrate that using a common annotation pipeline can result in different gene predictions, even for closely related cultivars, and repeat regions which collapse during assembly impact whole genome comparison. After accounting for differences in assembly and annotation, we demonstrate that the genome of Darmor-bzh contains a greater number of genes than the genome of Tapidor.
Date made available17 Mar 2017

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