Anatomical studies of leaflets of species from the plant subfamily Tribuloideae (Zygophyllaceae)

Leaflets of 26 accessions of species from subfamily Tribuloideae (Zygophyllaceae), using herbarium material, were sectioned after chemical fixation and embedding. For each section, the area of the mesophyll (M) tissue, bundle sheath (BS) tissue, and vascular tissue were measured, as well as the BS area, BS distance, and interveinal distance. The ratio of BS to M areas was also calculated. With a molecular phylogeny as a guide, these leaf anatomical traits, which are typically altered during the evolution of C4 photosynthesis, were further investigated. The timespan is 2017-04-01 to 2018-04-30.