A simple and innovative test for real-time detection of resistance in weeds

File list:
*A_simple_and_innovative_test_for_real_time_detection_of_resistance_in_weeds_R_Busi_21_2_23.xlsx
*dataRecord_agar_test.pdf

Methods for data generation and collection:
*Preparation of agar containing various herbicides used for annual ryegrass control
*Incubation of annual ryegrass seeds (susceptible and resistant populations) on agar under controlled and uncontrolled conditions
*Assessment of germination and seedling growth on herbicide-containing agar
*Assessment of a range of potential dormancy-breaking treatments
*Measurement of herbicide concentration in agar after various storage times
*Pot-based dose-response assays to compare results

Data processing: Microsoft Excel; R (drc package); Graph Pad Prism

Specific information needed to process data: N/A

Standards and calibration information: N/A

No. variables: 3 (herbicide treatment, incubation conditions, ryegrass population)

Experimental conditions: ambient laboratory temperature and light or 25/15 C alternating temperatures with 12 h photoperiod of white LED light (300 umol/m2/s)