3D X-ray microscopy scans from the article: "Sediment tolerance mechanisms identified in sponges using advanced imaging techniques."

  • Brian William Strehlow (Creator)
  • Mari Carmen Pineda (Creator)
  • Alan Duckworth (Creator)
  • Gary Kendrick (Creator)
  • Michael Renton (Creator)
  • Muhammad Azmi Abdul Wahab (Creator)
  • Nicole S. Webster (Creator)
  • Peta Clode (Creator)



These data are 3D X-ray microscopy scans of marine sponges exposed to sediments as well as scans of control individuals. The data are supplemental to the article "Sediment tolerance mechanisms identified in sponges using advanced imaging techniques." Published in PEERJ in 2017. The articles abstract is as follows: "Terrestrial runoff, resuspension events and dredging can affect filter-feeding sponges by elevating the concentration of suspended sediments, reducing light intensity, and smothering sponges with sediments. To investigate how sponges respond to pressures associated with increased sediment loads, the abundant and widely distributed Indo-Pacific species Ianthella basta was exposed to elevated suspended sediment concentrations, sediment deposition, and light attenuation for 48 hours (acute exposure) and 4 weeks (chronic exposure). In order to visualise the response mechanisms, sponge tissue was examined by 3D X-ray microscopy and scanning electron microscopy (SEM). Acute exposures resulted in sediment rapidly accumulating in the aquiferous system of I. basta, although this sediment was fully removed within 3 days. Sediment removal took longer (>2 weeks) following chronic exposures, and I. basta also exhibited tissue regression and a smaller aquiferous system. The application of advanced imaging approaches revealed that I. basta employs a multilevel system for sediment rejection and elimination, containing both active and passive components. Sponges responded to sediment stress through i) mucus production, ii) exclusion of particles by incurrent pores, iii) closure of oscula and pumping cessation, iv) expulsion of particles from the aquiferous system, and v) tissue regression to reduce the volume of the aquiferous system, thereby entering a dormant state. These mechanisms would result in tolerance and resilience to exposure to variable and high sediment loads associated with both anthropogenic impacts like dredging programs and natural pressures like flood events."
Date made available31 Oct 2017
PublisherThe University of Western Australia
Temporal coverageJun 2016 - Jul 2016
Date of data productionJul 2016 - Sept 2016
Geographical coverageNational Sea Simulator in Townsville, QLD, Australia; Fantome Island, Great Barrier Reef (GBR), Australia.
Geospatial point-18.683778, 146.511778Show on map

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