Description
Seminar Title:"Disordered regions determine protein phase separation to form condensed protein droplets"
Abstract:
Stable folded structures of proteins with complex amino acid sequences are generally well understood and are confidently predicted. However, many proteins are enriched in only a few amino acids – “low-complexity regions” (LCRs) – predicted to be intrinsically disordered. Relationships between LCR amino acid composition, protein structure, material state and function are poorly understood. Here, we focus on abundant RNA-binding proteins in mammalian cells (DBHS proteins), involved in transcription and RNA-processing. We show that LCRs are central for driving the organisation of molecules via protein condensation to form a distinct and separate liquid phase. While DBHS proteins have a conserved core folded dimerisation domain, their extended LCRs at each terminus are highly diverse, providing a unique opportunity for determining composition–function relationships of LCRs. Using in vitro biophysical assays and protein localisation studies in cells, we aim to understand how amino acid composition of LCRs determines emergent material state and function.
| Period | 2 Aug 2024 |
|---|---|
| Event title | Centre for Crop and Disease Management (CCDM) Seminar Series |
| Event type | Seminar |
| Location | Perth, Australia, Western AustraliaShow on map |
| Degree of Recognition | Local |